14. EVALUATION OF ANTI-ANXIETY ACTIVITY OF ASPARAGUS RACEMOSUS (SATAVARI) IN RATS BY USING LIGHT AND DARK MODEL
Present research work was performed to evaluate the anxiolytic activity of methanol extract of Asparagus racemosus (Satawari) in rat by using light and dark model. Anti-anxiety potentials of extracts of Asparagus racemosus were compared with diazepam. Rats of either sex were divided into seven groups of 6 animals in each group. Group were distilled water, vehicle medium as 10% carboxy methyl cellulose (C.M.C), diazepam 2mg/kg, extract of Asparagus racemosus (50mg/kg), extract of Asparagus racemosus (100mg/kg), extract of Asparagus racemosus (200mg/kg) and diazepam + effective dose of Asparagus racemosus (200 mg/kg). All the test solutions were freshly prepared daily and administered to animals for 7 days by oral route. After 7th day, each animal was checked to anxiety by using light and dark model. The extracts of Asparagus racemosus 100 mg/kg, 200 mg/kg and diazepam 2 mg/kg + Asparagus racemosus 200 mg/kg treated group shows significant (p<0.05) different reduced spend time in dark chamber of light and dark model as comparison with control group. Extracts of Asparagus racemosus 50mg/kg did not show significant (p<0.05) different reduced spend time in dark chamber of light and dark model as comparison with control group. Extract of Asparagus racemosus 200mg/kg with diazepam 2mg/kg gives synergetic anxiolytic activity in rats.