10. DEVELOPMENT AND VALIDATION OF CURRENT STATISTICAL APPROACH FOR ESTIMATING ACETAZOLAMIDE IN PHARMACEUTICAL DOSAGE FORM
In this work 3 very sensitive and effective (Zero order, AUC, HPLC and spectroscopy) has been developed to assess the Acetazolamide in bulk & pharmaceutical formulation. For the measurement of Acetazolamide in pharmaceutical formulations, a simple, precise and accurate Zero-order relative Spectroscopy approach was established and tested. Going to weigh 100 mg of Regular Acetazolamide in 10 ml volumetric flask with DMSO, prepared the stock solution. The final solution for stock was made with distilled water to produce 10000 mcg/ml. As per the protocol more dilutions have been prepared. The solution for the drug showed maximum absorbance at 262 nm. The linearity was observed within the range of concentrations of 100-600 mcg/ml. The Coefficient of Correlation was 0.999. It was noticed that the regression model is Y=0.0003c + 0.00075. The results were validated for predictability, accuracy, detection limit, linear range and roughness. The detection limit and quantitation limit for Acetazolamide estimation were found to be 2.34 (mcg/ml) and 20.65 (mcg/ml), respectively. Acetazolamide restoration was reported to be in the 89.0 percent range – 100 percent.